Description

Tissue DNA Purification Kit is designed for rapid purification of total DNA from a variety of tissues .. Sample is proteolitycally lysed in the presence of special buffer, aiding tissue and cells desintegration. Further, Proteinase K digests cellular proteins, including stripping-off DNA of all bound proteins, among them nucleases. Optimized buffer and ethanol are added to provide selective conditions for DNA binding during brief centrifugation, while contaminants pass through the ?resin in the spin-column. Traces of contaminants remaining on the resin are efficiently removed in two wash steps. High-quality cellular DNA is then eluted?with nuclease free water .