Description

The principle of ECLong is based on chemiluminescence
and is very convenient to detect the Horseradish peroxidase (HRP)
activity in many assays such as Western blotting, Southern blotting,
and Northern blotting. HRP catalyzes the chemiluminescent
oxidation of cyclic diacylhydrazides such as luminol by hydrogen
peroxide (H2O2). ECLong can enhance the luminol-dependent
chemiluminescence and be wildly used to detect the present of
HRP-conjugated antibodies or streptavidin which binding to antigen
or nucleotide sequence respectively.
Feature
? Signal Duration: 12 hours
? Detection Method: X-ray film or imaging acquisition system
? Suggested Antibody Dilution:
Primary: 1/1,000 ? 1/50,000 Secondary: 1/50,000 ? 1/250,000