Description

DNase I is a recombinant endonuclease that nonspecifically catalyzes the degradation of both single- and double-stranded DNA and DNA-RNA hybrids. The protease activity of DNase I endonuclease?has been eliminated; therefore, DNase I this enzyme is stable at its optimal (neutral) pH range and is suitable for RNA preparation at neutral pH. Recombinant DNase I (RNase-free) is an endonuclease that catalyzes, to the same degree, the random degradation of both single- and double-stranded DNA and produces 5′-P terminal oligonucleotides. Recombinant DNase I enzyme does not exhibit protease activity. DNase I is a glycosylated polypeptide which is commonly used for the degradation of single- and doublestranded DNAs (which are not required) into 5 phosphodinucleotide and oligonucleotide fragments. The activity of DNase I is dependent on the presence of bivalent metal ions. DNase I is an RNase-free glycoprotein which specifically degrades DNA. DNase I Solution is used for specific applications where integrity of RNA has to be maintained, e.g. for the removal of genomic DNA from RNA preparations prior to RT-PCR, during nick translations, for the isolation of DNAfree RNA after in vitro transcription reactions, DNase etc. DNaseI solution is also used for the prevention of clumping of concentrated or cryopreserved hematopoietic cell suspensions following thawing.