Description

? Broad range : 10-180 kDa
? 3 ?l or 5 ?l per loading for clear
visualization during electrophoresis on
15-well or 10-well mini-gel, respectively
? 2~3 ?l per well for general Western
transfering.
? Apply more for thicker (>1.5 mm) or larger
gel.
Description : The BlueRAY Prestained
Protein Ladder is designed for monitoring protein separated during
SDS polyacrylamide gel electrophoresis, verification of Western
transfer efficiency on membranes (PVDF, nylon or nitrocellulose) and
for approximate sizing of proteins Quality Control 5 ?l of BlueRAY
Prestained Protein Ladder resolves 10 bands in 4-20% SDS-PAGE
(Tris-glycine buffer) and after Western blotting to PVDF membrane
Contents : Approximately 0.2~0.4 mg/ml of each protein in buffer (20
mM Trisphosphate pH 7.5 at 25?C), 2% SDS, 0.2 mM Dithiothreitol,
3.6 M Urea, and 15% (v/v) Glycerol).