Description

HiTranScript? Reverse Transcriptase is a new reverse transcriptase based on M-MLV (RNase H-) Reverse Transcriptase through multiple mutations. HiTranScript? Reverse Transcriptase has the highest activity at 50?C and can withstand reaction temperatures up to 55?C, and can be used for reverse transcription of RNA templates with secondary structure. By enhancing the affinity with the template, more full-length cDNA can be obtained, and the total RNA can be detected as low as 1 pg. HiTranScript? Reverse Transcriptase is especially suitable for reverse transcription of a small number of templates and low-copy genes. In addition, the mismatch rate of HiTranScript? Reverse Transcriptase is also lower than that of M-MLV (RNase H-) Reverse Transcriptase, which improves the fidelity of cloning. Tolerate a reaction temperature of 50?C, suitable for RNA templates with secondary structure;
The template has strong affinity, and more full-length cDNA can be obtained;
Highly sensitive, can detect as little as 10 pg of total RNA